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Home > Product > Antibody > ATG5 mouse Monoclonal Antibody(3C7)

ATG5 mouse Monoclonal Antibody(3C7)

ATG5 mouse Monoclonal Antibody(3C7)

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Mouse, Rat

Applications: IF, ICC, WB, IHC-p, IP

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Mouse,Rat

Applications: IF, ICC, WB, IHC-p, IP

Product overview

Full product name

Autophagy protein 5

Code

BT-MCA0008

Host

Mouse

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Monoclonal

Immunogen

Recombinant Protein of ATG5

Purification

The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene.

Uniprot accession

Molecular weight

N/A

Gene ID

Human: 9494

Synonyms

Autophagy protein 5; APG5-like; Apoptosis-specific protein; ATG5; APG5L; ASP

Research area

Cell biology; Cancer; Metabolism

Target protein

ATG5

Recommended dilution

Application Dilution
IF 1:50-200
IHC-p 1:50-300
IP 1:50-200
WB 1:500-2000

Images

Immunofluorescence analysis of Hela cell. CREB-1 Polyclonal Antibody(red) was diluted at 1:200(4°C overnight). ATG5 mouse Monoclonal antibody(3C7)(green) was diluted at 1:200(4°C overnight).
Immunohistochemical analysis of paraffin-embedded Human-breast tissue. 1.ATG5 Mouse Monoclonal antibody(3C7) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1.ATG5 Mouse Monoclonal antibody(3C7) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human Ovarian Carcinoma Tissue using ATG5 Mouse Monoclonal antibody diluted at 1:200.
Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using ATG5 Mouse Monoclonal antibody diluted at 1:200.
Western blot analysis of Hela Cell Lysate using ATG5 Mouse Monoclonal antibody diluted at 1:10000

Protocols

Customer reviews and Q&As

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Product size

Price

  • 20ul

    $108.00

  • 50ul

    $180.00

  • 100ul

    $275.00

Add to Cart

Lead time: Within one week

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