Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > Antibody > Bax mouse Monoclonal Antibody(6F11)

Bax mouse Monoclonal Antibody(6F11)

Bax mouse Monoclonal Antibody(6F11)

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Rat, Mouse, Chicken

Applications: IF, ICC, WB, IHC-p

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Rat,Mouse,Chicken

Applications: IF, ICC, WB, IHC-p

Product overview

Full product name

BAX

Code

BT-MCA0010

Host

Mouse

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Monoclonal

Immunogen

Synthetic Peptide of Bax at AA range of 30-110

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

The protein encoded by BAX (BCL2 associated X, apoptosis regulator) belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for BAX.

Uniprot accession

Molecular weight

N/A

Gene ID

Human: 581

Synonyms

BAX

Research area

Cell biology; Cancer; Metabolism

Target protein

BAX

Recommended dilution

Application Dilution
IF 1:50-200
IHC 1:100-200
WB 1:1000-2000

Images

Western blot analysis of lysates from 1) Hela Cell Lysate, 2) C2C12 Cell Lysate, 3) PC12 Cell Lysate cells, (Green) primary antibody was diluted at 1:1000, 4°overnight,Dylight 800 secondary antibodywas diluted at 1:10000, 37°C 1hour. (Red) Actin Beta Polyclonal Antibody antibody was diluted at 1:5000 as loading control, 4°C overnight,Dylight 680 secondary antibody was diluted at 1:10000, 37°C 1hour.
Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1.Bax Mouse Monoclonal antibody(6F11) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1.Bax Mouse Monoclonal antibody(6F11) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using Bax Mouse Monoclonal antibody diluted at 1:200.
Immunohistochemical analysis of paraffin-embedded Mouse Kidney Tissue using Bax Mouse Monoclonal antibody diluted at 1:200.
Immunohistochemical analysis of paraffin-embedded Rat Testis Tissue using Bax Mouse Monoclonal antibody diluted at 1:200.
Western blot analysis of 1) Hela Cell Lysate, 2) C2C12 Cell Lysate, 3) PC12 Cell Lysate using Bax Mouse Monoclonal antibody diluted at 1:1000.
Western Blot analysis of chicken cell lysis using Antibody diluted at 1:1000

Protocols

Customer reviews and Q&As

No reviews
No reviews

Product size

Price

  • 20ul

    $108.00

  • 50ul

    $180.00

  • 100ul

    $275.00

Add to Cart

Lead time: Within one week

We are continuously updating our Antibody catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up