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Home > Product > Antibody > Bcl-2 Monoclonal Antibody(6B5)

Bcl-2 Monoclonal Antibody(6B5)

Bcl-2 Monoclonal Antibody(6B5)

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Mouse, Rat, chicken

Applications: IF, ICC, WB, IHC-p

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Mouse,Rat,chicken

Applications: IF, ICC, WB, IHC-p

Product overview

Full product name

Apoptosis regulator Bcl-2

Code

BT-MCA0011

Host

Mouse

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Monoclonal

Immunogen

Synthetic Peptide of Bcl-2

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Concentration

1 mg/ml

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Storage

-20°C for one year

Uniprot accession

Molecular weight

26266

Gene ID

Human: 596; Mouse: 12043; Rat: 24224

Synonyms

BCL2; Apoptosis regulator Bcl-2

Research area

Cell biology; Cancer; Metabolism; Signal transduction

Target protein

BCL2

Recommended dilution

Application Dilution
IF 1:50-200
IHC 1:200
WB 1:1000~2000

Images

Immunofluorescence analysis of Hela cell. Dnmt3b Polyclonal Antibody(red) was diluted at 1:200(4°C overnight). Bcl-2 Monoclonal antibody(6B5)(green) was diluted at 1:200(4°C overnight).
Western blot analysis of lysates from 1)Hela, 2) MCF-7 cells, (Green) primary antibody was diluted at 1:1000, 4°overnight, secondary antibody was diluted at 1:10000, 37°C 1hour. (Red) Actin Beta Polyclonal Antibody antibody was diluted at 1:5000 as loading control, 4°C overnight,Dylight 680 secondary antibody was diluted at 1:10000, 37°C 1hour.
Western blot analysis of Hela diluted at 1:1000.
Western Blot analysis of chicken cell lysis using Antibody diluted at 1:1000.
Immunohistochemical analysis of paraffin-embedded Human breast cancer. 1. Using Bcl-2 Mouse Monoclonal antibody (YM3041) diluted at 1:200 (4°C overnight).2.High-pressure and temperature Citric acid, pH6.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature, 50min). Picture was kindly provided by our customer from Tianjin Medical University Cancer Institute and Hospital
Immunohistochemical analysis of paraffin-embedded Human Lymph gland.1.Antibody was diluted at 1:100(4°C overnight). 2.High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human Lymph gland.1.Antibody was diluted at 1:100(4°C overnight). 2.High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human Lymph gland.1.Antibody was diluted at 1:100(4°C overnight). 2.High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human Lymph gland.1.Antibody was diluted at 1:200(4°C overnight). 2.High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human Lymph gland.1.Antibody was diluted at 1:200(4°C overnight). 2.High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human Lymph gland.1.Antibody was diluted at 1:200(4°C overnight). 2.High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human Fallopian tube.1.Antibody was diluted at 1:100(4°C overnight). 2.High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human Fallopian tube.1.Antibody was diluted at 1:100(4°C overnight). 2.High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human Fallopian tube.1.Antibody was diluted at 1:100(4°C overnight). 2.High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human Fallopian tube.1.Antibody was diluted at 1:200(4°C overnight). 2.High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human Fallopian tube.1.Antibody was diluted at 1:400(4°C overnight). 2.High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human Fallopian tube.1.Antibody was diluted at 1:400(4°C overnight). 2.High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human Fallopian tube.1.Antibody was diluted at 1:400(4°C overnight). 2.High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature, 30min).

Protocols

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Product size

Price

  • 20ul

    $108.00

  • 50ul

    $180.00

  • 100ul

    $275.00

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Lead time: Within one week

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