Immunofluorescence analysis of Hela cell. eIF2Alpha Polyclonal Antibody(red) was diluted at 1:200(4°C overnight). Caspase 9 Monoclonal antibody(3-20)(green) was diluted at 1:200(4°C overnight).
Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1.Caspase 9 Monoclonal antibody(3-20) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1.Caspase 9 Monoclonal antibody(3-20) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1.Caspase 9 Monoclonal antibody(3-20) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of Human-appendix tissue. 1.Caspase 9 Monoclonal antibody(3-20)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of Mouse-brain tissue. 1.Caspase 9 Monoclonal antibody(3-20)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of Rat-spleen tissue. 1.Caspase 9 Monoclonal antibody(3-20)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Western blot analysis of Hela diluted at 1) 1:2000 2) 1:5000
Western Blot analysis of chicken cell lysis using Antibody diluted at 1:1000.
Western blot analysis of lysates from 1) Hela, 2) Jurkat, 3)3T3 cells, (Green) primary antibody was diluted at 1:1000, 4°overnight, secondary antibody was diluted at 1:10000, 37°C 1hour. (Red) GAPDH Polyclonal Antibody antibody was diluted at 1:5000 as loading control, 4°C overnight,secondary antibody was diluted at 1:10000, 37°C 1hour.
