CHOP mouse Monoclonal Antibody(2B1)

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Blood coagulation
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Home > Product > Antibody > CHOP mouse Monoclonal Antibody(2B1)

CHOP mouse Monoclonal Antibody(2B1)

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Rat, Mouse

Applications: WB, IF, ICC, IHC-p

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Rat,Mouse

Applications: WB, IF, ICC, IHC-p

Product overview

Full product name

DDIT3

Code

BT-MCA0020

Host

Mouse

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Monoclonal

Immunogen

Synthetic Peptide of CHOP at AA range of 10-90

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified.

Uniprot accession

Human:;Mouse:;Rat:;

Molecular weight

N/A

Gene ID

Human: 1649

Synonyms

DDIT3

Research area

Epigenetics and nuclear signaling; Stem cells; Developmental biology; Metabolism

Target protein

DDIT3

Recommended dilution

Application	Dilution
IF	1:200
IHC	1:100-200
WB	1:1000-2000

Images

Immunofluorescence analysis of Hela cell. Calnexin Polyclonal Antibody(green) was diluted at 1:200(4°C overnight). (red) was diluted at 1:200(4°C overnight).

Immunohistochemical analysis of paraffin-embedded Human-stomach tissue. 1.CHOP Mouse Monoclonal antibody(2B1) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunofluorescence analysis of Mouse-brain tissue. 1.CHOP Mouse Monoclonal antibody(2B1)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunohistochemical analysis of paraffin-embedded Human Stomach Carcinoma Tissue using CHOP Mouse Monoclonal antibody diluted at 1:200.

Immunohistochemical analysis of paraffin-embedded Human Pancreas Carcinoma Tissue using CHOP Mouse Monoclonal antibody diluted at 1:200.

Western blot analysis of Mouse Liver Tissue Lysate using CHOP Mouse Monoclonal antibody diluted at 1:2000.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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