Collagen III Monoclonal Antibody(Q76)

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Home > Product > Antibody > Collagen III Monoclonal Antibody(Q76)

Collagen III Monoclonal Antibody(Q76)

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, IF, ICC

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Mouse,Rat

Applications: WB, IHC-p, IF, ICC

Product overview

Full product name

Collagen alpha-1(III) chain

Code

BT-MCA0025

Host

Mouse

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Monoclonal

Immunogen

Synthetic Peptide of Collagen III

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Concentration

1 mg/ml

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Storage

-20°C for one year

Background

This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.

Uniprot accession

Human:;Mouse:;Rat:;

Molecular weight

138564

Gene ID

Human: 1281; Mouse: 12825; Rat: 84032

Synonyms

COL3A1; Collagen alpha-1(III) chain

Research area

Signal transduction; Stem cells

Target protein

COL3A1

Recommended dilution

Application	Dilution
IF	1:200
IHC	1:50-300
WB	1:1000

Images

Western blot analysis of lysates from 1) MCF7 , 2) Hela, 3) 293T cells, (Green) primary antibody was diluted at 1:1000, 4°overnight, secondary antibody was diluted at 1:10000, 37°C 1hour. (Red) Actin Beta Polyclonal Antibody antibody was diluted at 1:5000 as loading control, 4°C overnight,secondary antibody was diluted at 1:10000, 37°C 1hour.

Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1.Collagen III Monoclonal antibody(Q76) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1.Collagen III Monoclonal antibody(Q76) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1.Collagen III Monoclonal antibody(Q76) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunofluorescence analysis of Mouse-liver tissue. 1.Collagen III Monoclonal antibody(Q76)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Western blot analysis of Hela diluted at 1:1000.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

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Lead time: Within one week

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