Immunofluorescence analysis of Hela cell. c-Myc Polyclonal Antibody(red) was diluted at 1:200(4°C overnight). CYCS Monoclonal antibody(4B10)(green) was diluted at 1:200(4°C overnight).
Western blot analysis of lysates from 1) Hela, 2) 293T, 3) 3T3. cells, (Green) primary antibody was diluted at 1:1000, 4°overnight, secondary antibody was diluted at 1:10000, 37°C 1hour. (Red) GAPDH Polyclonal Antibody antibody was diluted at 1:5000 as loading control, 4°C overnight,secondary antibody was diluted at 1:10000, 37°C 1hour.
Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1.CYCS Monoclonal antibody(4B10) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1.CYCS Monoclonal antibody(4B10) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1.CYCS Monoclonal antibody(4B10) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of Human-liver-cancer tissue. 1.CYCS Monoclonal antibody(4B10)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of Mouse-spleen tissue. 1.CYCS Monoclonal antibody(4B10)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of Rat-liver tissue. 1.CYCS Monoclonal antibody(4B10)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Western blot analysis of 1) Hela, 2) 293T, 3) 3T3. 4) Mouse Liver Tissue, 5) Rat Liver Tissue, 6) Rat Kidney Tissue using CYCS Monoclonal antibody.
Immunohistochemical analysis of paraffin-embedded human Breast caricnoma using CYCS Monoclonal antibody.
Western Blot analysis of chicken cell lysis using Antibody diluted at 1:1000.
