EFHD1 Monoclonal Antibody(3G2)

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Home > Product > Antibody > EFHD1 Monoclonal Antibody(3G2)

EFHD1 Monoclonal Antibody(3G2)

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, IF, ICC

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Mouse,Rat

Applications: WB, IHC-p, IF, ICC

Product overview

Full product name

EF-hand domain-containing protein D1

Code

BT-MCA0032

Host

Mouse

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Monoclonal

Immunogen

Synthetic Peptide of EFHD1

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Concentration

1 mg/ml

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Storage

-20°C for one year

Background

This gene encodes a member of the EF-hand super family of calcium binding proteins, which are involved in a variety of cellular processes including mitosis, synaptic transmission, and cytoskeletal rearrangement. The protein encoded by this gene is composed of an N-terminal disordered region, proline-rich elements, two EF-hands, and a C-terminal coiled-coil domain. This protein has been shown to associate with the mitochondrial inner membrane, and in HeLa cells, acts as a novel mitochondrial calcium ion sensor for mitochondrial flash activation. Alternative splicing results in multiple transcript variants.

Uniprot accession

Human:;Mouse:;

Molecular weight

N/A

Gene ID

Human: 80303; Mouse: 98363

Synonyms

EF-hand domain-containing protein D1 (EF-hand domain-containing protein 1) (Swiprosin-2)

Research area

Cell Biology

Target protein

EFHD1

Recommended dilution

Application	Dilution
IF	1:100-200
IHC	1:50-300
WB	1:2000

Images

Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1.EFHD1 Monoclonal antibody(3G2) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1.EFHD1 Monoclonal antibody(3G2) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1.EFHD1 Monoclonal antibody(3G2) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunofluorescence analysis of Mouse-lung tissue. 1.EFHD1 Monoclonal antibody(3G2)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Western blot analysis of 1) Mouse spleen tissue, 2) Rat spleen tissue diluted at 1:3000.

IF analysis of Hela with antibody (Left) and DAPI (Right) diluted at 1:100.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

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Lead time: Within one week

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