HAO1 Monoclonal Antibody(Mix)

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Home > Product > Antibody > HAO1 Monoclonal Antibody(Mix)

HAO1 Monoclonal Antibody(Mix)

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity:Mouse, Rat

Applications: WB, IHC-p, IF, ICC

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity: Mouse,Rat

Applications: WB, IHC-p, IF, ICC

Product overview

Full product name

Hydroxyacid oxidase 1

Code

BT-MCA0044

Host

Mouse

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Monoclonal

Immunogen

Recombinant Protein of HAO1

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Storage

-20°C for one year

Background

This gene is one of three related genes that have 2-hydroxyacid oxidase activity yet differ in encoded protein amino acid sequence, tissue expression and substrate preference. Subcellular location of the encoded protein is the peroxisome. Specifically, this gene is expressed primarily in liver and pancreas and the encoded protein is most active on glycolate, a two-carbon substrate. The protein is also active on 2-hydroxy fatty acids. The transcript detected at high levels in pancreas may represent an alternatively spliced form or the use of a multiple near-consensus upstream polyadenylation site.

Uniprot accession

Human:;Mouse:;

Molecular weight

N/A

Gene ID

Human: 54363

Synonyms

Hydroxyacid oxidase 1; HAOX1; Glycolate oxidase; GOX

Research area

Tags & Cell Markers; Signal Transduction; Cardiovascular; Metabolism

Target protein

HAO1

Recommended dilution

Application	Dilution
IF	1:200
IHC	1:50-300
WB	1:1000-2000

Images

Immunofluorescence analysis of Hela cell. Chk2 (phospho Thr68) Polyclonal Antibody(red) was diluted at 1:200(4°C overnight). HAO1 Monoclonal antibody(Mix)(green) was diluted at 1:200(4°C overnight).

Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1.HAO1 Monoclonal antibody(Mix) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-liver tissue. 1.HAO1 Monoclonal antibody(Mix) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1.HAO1 Monoclonal antibody(Mix) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunofluorescence analysis of Human-appendix tissue. 1.HAO1 Monoclonal antibody(Mix)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of Mouse-spleen tissue. 1.HAO1 Monoclonal antibody(Mix)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Western blot analysis of 1) Mouse Liver Tissue, 2) Rat Liver Tissue using HAO1 Monoclonal antibody.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

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Lead time: Within one week

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