Histone H2B Monoclonal Antibody(Mix)

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Home > Product > Antibody > Histone H2B Monoclonal Antibody(Mix)

Histone H2B Monoclonal Antibody(Mix)

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Mouse, Rat

Applications: WB, IF, ICC,

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Mouse,Rat

Applications: WB, IF, ICC,

Product overview

Full product name

Histone H2B type 1-B

Code

BT-MCA0047

Host

Mouse

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Monoclonal

Immunogen

Synthetic Peptide of Histone H2B

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Concentration

1 mg/ml

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Storage

-20°C for one year

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails| instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

Uniprot accession

Human:;Mouse:;

Molecular weight

13950

Gene ID

Human: 3018; Mouse: 319178

Synonyms

HIST1H2BB; H2BFF; Histone H2B type 1-B; Histone H2B.1; Histone H2B.f; H2B; f

Research area

Epigenetics and nuclear signaling

Target protein

HIST1H2BB

Recommended dilution

Application	Dilution
IF	1:200
WB	1:1000-3000

Images

Immunofluorescence analysis of Hela cell. Brp44L Polyclonal Antibody(green) was diluted at 1:200(4°C overnight). (red) was diluted at 1:200(4°C overnight).

Immunofluorescence analysis of Human-liver-cancer tissue. 1.Histone H2B Monoclonal antibody(Mix)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of Mouse-kidney tissue. 1.Histone H2B Monoclonal antibody(Mix)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of Rat-heart tissue. 1.Histone H2B Monoclonal antibody(Mix)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Western blot analysis of 1) Hela, 2) 3T3. 3) Raw264.7, 4) Rat Brain, 5) Rat Kidney diluted at 1:2000.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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