Histone H3 Monoclonal Antibody(1G1)

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Home > Product > Antibody > Histone H3 Monoclonal Antibody(1G1)

Histone H3 Monoclonal Antibody(1G1)

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Mouse, Rat, Yeast

Applications: WB, IHC-p, IF, ICC, IP

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Mouse,Rat,Yeast

Applications: WB, IHC-p, IF, ICC, IP

Product overview

Full product name

Histone H3.1

Code

BT-MCA0049

Host

Mouse

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Monoclonal

Immunogen

Recombinant Protein of Histone H3

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Concentration

1 mg/ml

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Storage

-20°C for one year

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails| instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

Uniprot accession

Human:;P84245:;

Molecular weight

15273

Gene ID

Human: 8350; 8351; 8352; 8353; 8354; 8355; 8356; 8357; 8358; 8968; Mouse: 319152; 15077; 15078; Rat: 291159; 100361558

Synonyms

HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3; a; Histone H3; b; Histone H3; c; Histone H3; d; Histone H3; f; Histone H3; h; Histone H3; i; Histone H3; j; Histone H3; k; Histone H3; l; HIST2H3A; HIST2H3C; H3F2; H3FM; HIST2H3D; Histone H3.2; Histone H3; m; Histone H3; o; H3F3A; H3.3A; H3F3; PP781; H3F3B; H3.3B; Histone H3.3

Research area

Epigenetics and nuclear signaling

Target protein

H31

Recommended dilution

Application	Dilution
IF	1:100-500
IHC	1:50-300
IP	1:200
WB	1:2000-5000

Images

Immunofluorescence analysis of Hela cell. Amyloid-Beta Polyclonal Antibody(green) was diluted at 1:200(4°C overnight). (red) was diluted at 1:200(4°C overnight).

Western blot analysis of lysates from Hela, Raw, Mouse Brain Tissue and Rat Brain Tissue cells, (Green) primary antibody was diluted at 1:1000, 4°overnight, Dylight 800 secondary antibody was diluted at 1:10000, 37°C 1hour. (Red) Actin Beta Polyclonal Antibody antibody was diluted at 1:5000 as loading control, 4°C overnight,Dylight 680 secondary antibody was diluted at 1:10000, 37°C 1hour.

Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1.Histone H3 Monoclonal antibody(1G1) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1.Histone H3 Monoclonal antibody(1G1) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1.Histone H3 Monoclonal antibody(1G1) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunofluorescence analysis of Human-liver-cancer tissue. 1.Histone H3 Monoclonal antibody(1G1)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of Mouse-liver tissue. 1.Histone H3 Monoclonal antibody(1G1)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of Rat-liver tissue. 1.Histone H3 Monoclonal antibody(1G1)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Western blot analysis of 1) Hela, 2) Raw, 3) Mouse Brain Tissue, 4) Rat Brain Tissue diluted at 1:5000.

IF analysis of Hela diluted at 1:200.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

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Lead time: Within one week

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