HP-1 Alpha mouse Monoclonal Antibody(5E3)

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Blood coagulation
Metabolism
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Neuroscience
Cell biology
Signal transduction
Developmental biology
Stem cells
Epigenetics and nuclear signaling
Tags & cell markers
Immunology

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Home > Product > Antibody > HP-1 Alpha mouse Monoclonal Antibody(5E3)

HP-1 Alpha mouse Monoclonal Antibody(5E3)

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Mouse, Rat

Applications: IF, ICC, WB, IHC-p

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Mouse,Rat

Applications: IF, ICC, WB, IHC-p

Product overview

Full product name

Chromobox protein homolog 5

Code

BT-MCA0050

Host

Mouse

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Monoclonal

Immunogen

Recombinant Protein of HP-1α

Purification

The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Uniprot accession

Human:;Mouse:;

Molecular weight

N/A

Gene ID

Human: 23468

Synonyms

Chromobox protein homolog 5; Antigen p25; Heterochromatin protein 1 homolog alpha; HP1 alpha

Research area

Epigenetics and nuclear signaling; Tags & cell markers

Target protein

CBX5 HP1A

Recommended dilution

Application	Dilution
IF	1:50-200
IHC-p	1:50-300
WB	1:500-2000

Images

Immunofluorescence analysis of human-uterus tissue. 1.HP-1Alpha Mouse Monoclonal antibody(5E3)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1.HP-1Alpha Mouse Monoclonal antibody(5E3) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1.HP-1Alpha Mouse Monoclonal antibody(5E3) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using HP-1 Alpha Mouse Monoclonal antibody diluted at 1:200

Immunohistochemical analysis of paraffin-embedded Human Placenta Tissue using HP-1 Alpha Mouse Monoclonal antibody diluted at 1:200

Western blot analysis of 1) Hela Cell Lysate, 2)3T3 Cell Lysate, 3) PC12 Cell Lysate using HP-1GammaAlpha Mouse Monoclonal antibody diluted at 1:1000.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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