HP-1Gamma mouse Monoclonal Antibody(2F5)

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Home > Product > Antibody > HP-1Gamma mouse Monoclonal Antibody(2F5)

HP-1Gamma mouse Monoclonal Antibody(2F5)

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Mouse, Rat

Applications: IF, ICC, WB, IHC-p

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Mouse,Rat

Applications: IF, ICC, WB, IHC-p

Product overview

Full product name

Chromobox protein homolog 3

Code

BT-MCA0051

Host

Mouse

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Monoclonal

Immunogen

Recombinant Protein of HP-1γ

Purification

The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.

Uniprot accession

Human:;Mouse:;

Molecular weight

N/A

Gene ID

Human: 11335

Synonyms

Chromobox protein homolog 3; HECH; Heterochromatin protein 1 homolog gamma; HP1 gamma; Modifier 2 protein

Research area

Others

Target protein

CBX3

Recommended dilution

Application	Dilution
IF	1:50-200
IHC-p	1:50-300
WB	1:500-2000

Images

Immunofluorescence analysis of mouse-spleen tissue. 1.HP-1Gamma Mouse Monoclonal antibody(2F5)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1.HP-1Gamma Mouse Monoclonal antibody(2F5) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1.HP-1Gamma Mouse Monoclonal antibody(2F5) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using HP-1Gamma Mouse Monoclonal antibody diluted at 1:200

Immunohistochemical analysis of paraffin-embedded Human Placenta Tissue using HP-1Gamma Mouse Monoclonal antibody diluted at 1:200

Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using HP-1Gamma Mouse Monoclonal antibody diluted at 1:200

Western blot analysis of 1) Hela Cell Lysate, 2)3T3 Cell Lysate, 3) PC12 Cell Lysate using HP-1Gamma Mouse Monoclonal antibody diluted at 1:1000.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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