Immunofluorescence analysis of Hela cell. AIM2 Polyclonal Antibody(red) was diluted at 1:200(4°C overnight). HSP70 Monoclonal antibody(3G10)(green) was diluted at 1:200(4°C overnight).
Western blot analysis of lysates from HT-29, NIH/3T3. and HepG2 cells, primary antibody was diluted at 1:1000, 4°C overnight, secondary antibody was diluted at 1:10000, 37°C 1hour.
Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1.HSP70 Monoclonal antibody(3G10) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1.HSP70 Monoclonal antibody(3G10) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1.HSP70 Monoclonal antibody(3G10) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of Human-breast-cancer tissue. 1.HSP70 Monoclonal antibody(3G10)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of Mouse-lung tissue. 1.HSP70 Monoclonal antibody(3G10)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of Rat-testis tissue. 1.HSP70 Monoclonal antibody(3G10)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Western blot analysis of 1) Hela, 2) Mouse Brain diluted at 1:2000.
IF analysis of Hela with antibody (Left) and DAPI (Right) diluted at 1:100.
Immunohistochemical analysis of paraffin-embedded Human Lung caricnoma using Mouse Monoclonal antibody diluted at 1:500.
Western blot analysis of Pig Skeletal Muscle with HSP70 Monoclonal antibody diluted at 1:2.000.
