LC3A mouse Monoclonal Antibody(5G10)

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Home > Product > Antibody > LC3A mouse Monoclonal Antibody(5G10)

LC3A mouse Monoclonal Antibody(5G10)

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Rat, Mouse

Applications: WB, IF, ICC, IHC-p

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Rat,Mouse

Applications: WB, IF, ICC, IHC-p

Product overview

Full product name

LC3A

Code

BT-MCA0058

Host

Mouse

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Monoclonal

Immunogen

Synthetic Peptide of LC3A

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis.

Uniprot accession

Human:;Mouse:;Rat:;

Molecular weight

N/A

Gene ID

Human: 84557

Synonyms

Microtubule-associated proteins 1A; 1B light chain 3A; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; MAP1 light chain 3-like protein 1; MAP1A; MAP1B light chain 3 A; MAP1A; MAP1B LC3 A; Microtubule-associated protein 1 light chain 3 alpha

Research area

Cardiovascular; Signal transduction; Metabolism; Cancer

Target protein

MAP1LC3A

Recommended dilution

Application	Dilution
IF	1:200
IHC	1:100-200
WB	1:1000-2000

Images

Immunofluorescence analysis of Hela cell. CREB-1 Polyclonal Antibody(red) was diluted at 1:200(4°C overnight). LC3A mouse Monoclonal antibody(5G10)(green) was diluted at 1:200(4°C overnight).

Western blot analysis of lysates from 1) Hela Cell Lysate, 2) 3T3 Cell Lysate, 3) Rat Brain Tissue cells, (Green) primary antibody was diluted at 1:1000, 4°overnight, Dylight 800 secondary antibody was diluted at 1:10000, 37°C 1hour. (Red) Actin Beta Polyclonal Antibody antibody was diluted at 1:5000 as loading control, 4°C overnight,Dylight 680 secondary antibody was diluted at 1:10000, 37°C 1hour.

Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1.LC3A Mouse Monoclonal antibody(5G10) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1.LC3A Mouse Monoclonal antibody(5G10) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunofluorescence analysis of Human-lung-cancer tissue. 1.LC3A Mouse Monoclonal antibody(5G10)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of Mouse-spleen tissue. 1.LC3A Mouse Monoclonal antibody(5G10)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunohistochemical analysis of paraffin-embedded Human Heptacarcinoma Tissue using LC3A Mouse Monoclonal antibody diluted at 1:200.

Immunohistochemical analysis of paraffin-embedded Human Prostate Carcinoma Tissue using LC3A Mouse Monoclonal antibody diluted at 1:200.

Western blot analysis of 1) Hela Cell Lysate, 2) 3T3 Cell Lysate, 3) Rat Brain Tissue Lysate using LC3A Mouse Monoclonal antibody diluted at 1:1000.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

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Lead time: Within one week

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