PCNA Monoclonal Antibody(12D10)

Product

OneStep ELISA kit
Primacu ELISA kit
Antibodies
Biochemicals
Recombinant proteins

Service

Custom ELISA assay services
Bulk quote

Research area

Blood coagulation
Metabolism
Cancer
Microbiology
Cardiovascular
Neuroscience
Cell biology
Signal transduction
Developmental biology
Stem cells
Epigenetics and nuclear signaling
Tags & cell markers
Immunology

Resources

Protocols
FAQs
Product brochure
Promotion
Troubleshooting guides
About ELISAs

About us

Company profile
Worldwide distributors
Manufacturing service

Home > Product > Antibody > PCNA Monoclonal Antibody(12D10)

PCNA Monoclonal Antibody(12D10)

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Rat, Mouse

Applications: WB, IF, ICC, IHC-p

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Rat,Mouse

Applications: WB, IF, ICC, IHC-p

Product overview

Full product name

Proliferating cell nuclear antigen

Code

BT-MCA0068

Host

Mouse

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Monoclonal

Immunogen

Synthetic Peptide of PCNA

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Concentration

1 mg/ml

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Storage

-20°C for one year

Background

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

Uniprot accession

Human:;Mouse:;Rat:;

Molecular weight

28769

Gene ID

Human: 5111; Mouse: 18538; Rat: 25737

Synonyms

PCNA; Proliferating cell nuclear antigen; PCNA; Cyclin

Research area

Cancer; Cell biology; Epigenetics and nuclear signaling; Tags & cell markers

Target protein

PCNA

Recommended dilution

Application	Dilution
IF	1:200
IHC	1:200
WB	1:5000

Images

Immunofluorescence analysis of Hela cell. 14-3-3 Theta/τ (phospho Ser232) Polyclonal Antibody(green) was diluted at 1:200(4°C overnight). (red) was diluted at 1:200(4°C overnight).

Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1.PCNA Monoclonal antibody(12D10) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1.PCNA Monoclonal antibody(12D10) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1.PCNA Monoclonal antibody(12D10) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunofluorescence analysis of Human-lung-cancer tissue. 1.PCNA Monoclonal antibody(12D10)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of Rat-testis tissue. 1.PCNA Monoclonal antibody(12D10)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Western blot analysis of Hela (1) Rat brain (2) NIH 3T3 (3) 293T (4) diluted at 1:5000.

Protocols

> Protocol Booklet

Customer reviews and Q&As

No reviews

Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

Custom quote | Bulk request

We are continuously updating our Antibody catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.