Survivin Monoclonal Antibody(Mix)

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Blood coagulation
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Home > Product > Antibody > Survivin Monoclonal Antibody(Mix)

Survivin Monoclonal Antibody(Mix)

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Rat

Applications: WB, IHC-p, IF

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Rat

Applications: WB, IHC-p, IF

Product overview

Full product name

Baculoviral IAP repeat-containing protein 5

Code

BT-MCA0074

Host

Mouse

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Monoclonal

Immunogen

Recombinant Protein of Survivin

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Storage

-20°C for one year

Background

This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors, yet low in adult tissues. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Uniprot accession

Human:;Mouse:;Rat:;

Molecular weight

N/A

Gene ID

Human: 332

Synonyms

BIRC5; API4; IAP4; Baculoviral IAP repeat-containing protein 5; Apoptosis inhibitor 4; Apoptosis inhibitor survivin

Research area

Cancer; Cell biology; Neuroscience

Target protein

BIRC5

Recommended dilution

Application	Dilution
IHC	1:200-500
WB	1:1000-2000

Images

Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1.Survivin Monoclonal antibody(Mix) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-spinal-cord tissue. 1.Survivin Monoclonal antibody(Mix) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue. 1.Survivin Monoclonal antibody(Mix) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Western blot analysis of 1) Hela, 2) 293. 3) PC12 using Survivin Monoclonal antibody.

Immunohistochemical analysis of paraffin-embedded human colon caricnoma using Survivin Monoclonal antibody.

Immunohistochemical analysis of paraffin-embedded Human breast cancer. 1. Using Survivin Mouse Monoclonal antibody (YM3419) diluted at 1:200 (4°C overnight).2.High-pressure and temperature Citric acid, pH6.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature, 50min). Picture was kindly provided by our customer from Tianjin Medical University Cancer Institute and Hospital

Immunohistochemical analysis of paraffin-embedded Human kidney.1.Antibody was diluted at 1:200(4°C overnight). 2.High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature, 30min).

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> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

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Lead time: Within one week

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