Alpha skeletal muscle actin Monoclonal Antibody(4B11)

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Home > Product > Antibody > Alpha skeletal muscle actin Monoclonal Antibody(4B11)

Alpha skeletal muscle actin Monoclonal Antibody(4B11)

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, IF, ICC, IP

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Mouse,Rat

Applications: WB, IHC-p, IF, ICC, IP

Product overview

Full product name

Actin alpha skeletal muscle

Code

BT-MCA0082

Host

Mouse

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Monoclonal

Immunogen

Synthetic Peptide of α skeletal muscle actin

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Concentration

1 mg/ml

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Storage

-20°C for one year

Background

The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.

Uniprot accession

Human:;Mouse:;Rat:;

Molecular weight

42051

Gene ID

Human: 58; Mouse: 11459; Rat: 29437

Synonyms

ACTA1; ACTA; Actin; alpha skeletal muscle; Alpha-actin-1

Research area

Cardiovascular; Signal transduction

Target protein

ACTA1

Recommended dilution

Application	Dilution
IF	1:200
IHC	1:50-300
IP	1:200
WB	1:500-10000

Images

Immunohistochemical analysis of paraffin-embedded Rat-liver tissue. 1.Alpha skeletal muscle actin Monoclonal antibody(4B11) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1.Alpha skeletal muscle actin Monoclonal antibody(4B11) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunofluorescence analysis of Human-liver-cancer tissue. 1.Alpha skeletal muscle actin Monoclonal antibody(4B11)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of Mouse-liver tissue. 1.Alpha skeletal muscle actin Monoclonal antibody(4B11)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of Rat-liver tissue. 1.Alpha skeletal muscle actin Monoclonal antibody(4B11)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Western blot analysis of 1) Hela, 2) Mouse Brain tissue, 3) Rat Brain tissue diluted at 1:20000.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

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Lead time: Within one week

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