Beta-actin Monoclonal Antibody(5B7)

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Home > Product > Antibody > Beta-actin Monoclonal Antibody(5B7)

Beta-actin Monoclonal Antibody(5B7)

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, Insect

Applications: IF, ICC, WB, IHC-p

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Rat,Mouse,Monkey,Dog,Chicken,Hamster,Rabbit,Insect

Applications: IF, ICC, WB, IHC-p

Product overview

Full product name

Actin cytoplasmic 1

Code

BT-MCA0088

Host

Mouse

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Monoclonal

Immunogen

Synthetic Peptide of Beta-actin

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Concentration

1 mg/ml

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Storage

-20°C for one year

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.

Uniprot accession

Human:;Mouse:;Rat:;

Molecular weight

41737

Gene ID

Human: 60; Mouse: 11461; Rat: 81822

Synonyms

ACTB; Actin; cytoplasmic 1; Beta-actin; Actin Beta; Beta-Actin; Beta actin

Research area

Tags & cell markers; Signal transduction

Target protein

ACTB

Recommended dilution

Application	Dilution
IF	1:50-200
IHC	1:200-300
WB	1:5000-20000

Images

Immunofluorescence analysis of Hela cell. Cyclin A Polyclonal Antibody(red) was diluted at 1:200(4°C overnight). Beta-actin Monoclonal antibody(5B7)(green) was diluted at 1:200(4°C overnight).

Western blot analysis of 1 HEK293 2 SW480 3 HEPG2 4 MCF-7 5 mouse brain 6 Rat brain 7 Hela 8 A549 lysates, primary antibody was diluted at 1:5000, 4°C overnight, secondary antibody was diluted at 1:10000, 37°C 1hour.

Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1.Beta-actin Monoclonal antibody(5B7) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1.Beta-actin Monoclonal antibody(5B7) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1.Beta-actin Monoclonal antibody(5B7) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Western blot analysis of Hela (1) Rat brain (2) Mouse brain (3) Chicken lung (4) and Rabbit testis (5) diluted at 1:10000.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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