Beta-Tubulin Monoclonal Antibody(5G3)

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Home > Product > Antibody > Beta-Tubulin Monoclonal Antibody(5G3)

Beta-Tubulin Monoclonal Antibody(5G3)

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, sheep, Insect, Yeast, Fish

Applications: WB, IF, ICC, IHC-p

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Rat,Mouse,Monkey,Dog,Chicken,Hamster,Rabbit,sheep,Insect,Yeast,Fish

Applications: WB, IF, ICC, IHC-p

Product overview

Full product name

Tubulin beta-3 chain

Code

BT-MCA0089

Host

Mouse

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Monoclonal

Immunogen

Synthetic Peptide of Beta-Tubulin

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Concentration

1 mg/ml

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Storage

-20°C for one year

Background

This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6.

Uniprot accession

Human:;Mouse:;Rat:;

Molecular weight

50433

Gene ID

Human: 10381; Mouse: 22152; Rat: 246118

Synonyms

TUBB3; TUBB4; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-III

Research area

Tags & cell markers; Signal transduction

Target protein

TUBB3

Recommended dilution

Application	Dilution
IF	1:200
IHC	1:200
WB	1:5000

Images

Immunofluorescence analysis of Hela cell. CREB-1 (phospho Ser133) Polyclonal Antibody(red) was diluted at 1:200(4°C overnight). Beta-Tubulin Monoclonal antibody(5G3)(green) was diluted at 1:200(4°C overnight).

Immunohistochemical analysis of paraffin-embedded Human-colon tissue. 1.Beta-Tubulin Monoclonal antibody(5G3) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1.Beta-Tubulin Monoclonal antibody(5G3) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1.Beta-Tubulin Monoclonal antibody(5G3) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunofluorescence analysis of Human-appendix tissue. 1.Beta-Tubulin Monoclonal antibody(5G3)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of Mouse-lung tissue. 1.Beta-Tubulin Monoclonal antibody(5G3)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Western blot analysis of A549 (1) Rat brain (2) Mouse brain (3) Chicken lung (4) and Rabbit testis (5) Sheep muscle (6) diluted at 1:5000.

IF analysis of Hela with Beta-Tubulin Monoclonal antibody(Left) and DAPI (Right) diluted at 1:100.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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