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Home > Product > Antibody > DDX4 Monoclonal Antibody

DDX4 Monoclonal Antibody

DDX4 Monoclonal Antibody

Product Summary

Size: 50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity:Human

Applications: WB, IHC-p, IF, ICC, FCM, ELISA

Product Summary

Size: 50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Applications: WB, IHC-p, IF, ICC, FCM, ELISA

Product overview

Full product name

Probable ATP-dependent RNA helicase DDX4

Code

BT-MCA0440

Host

Mouse

Isotype

IgG

Size

50ul,100ul

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human DDX4 expressed in E. Coli

Purification

Affinity purification

Concentration

1 mg/ml

Formulation

Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol.

Storage

-20°C for one year

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene.

Uniprot accession

Molecular weight

N/A

Gene ID

Human: 54514

Synonyms

DDX4; VASA; Probable ATP-dependent RNA helicase DDX4; DEAD box protein 4; Vasa homolog

Research area

Epigenetics and nuclear signaling; Stem cells; Developmental biology

Target protein

DDX4

Recommended dilution

Application Dilution
FC 1:200 - 1:400
IF 1:200 - 1:1000
IHC 1:200 - 1:1000
WB 1:500 - 1:2000

Images

Western Blot analysis using DDX4 Monoclonal antibody against DDX4-hIgGFc transfected HEK293 cell lysate.
Immunohistochemistry analysis of paraffin-embedded human lung cancer (A) and rectal cancer (B), showing cytoplasmic localization with DAB staining using DDX4 Monoclonal antibody.
Immunofluorescence analysis of MSCs(left) and NTERA-2 (right) cells using DDX4 Monoclonal antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin.
Flow cytometric analysis of MSCS cells using DDX4 Monoclonal antibody (green) and negative control (purple).

Protocols

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Product size

Price

  • 50ul

    $180.00

  • 100ul

    $300.00

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Lead time: Within one week

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