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Home > Product > Antibody > Smad5 Monoclonal Antibody

Smad5 Monoclonal Antibody

Smad5 Monoclonal Antibody

Product Summary

Size: 50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Rat

Applications: WB, IHC-p, IF, ICC, FCM, ELISA

Product Summary

Size: 50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Rat

Applications: WB, IHC-p, IF, ICC, FCM, ELISA

Product overview

Full product name

Mothers against decapentaplegic homolog 5

Code

BT-MCA1137

Host

Mouse

Isotype

IgG

Size

50ul,100ul

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human Smad5 expressed in E. Coli.

Purification

Affinity purification

Concentration

1 mg/ml

Formulation

Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol.

Storage

-20°C for one year

Background

The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants.

Uniprot accession

Molecular weight

N/A

Gene ID

Human: 409059328

Synonyms

SMAD5; MADH5; Mothers against decapentaplegic homolog 5; MAD homolog 5; Mothers against DPP homolog 5; JV5-1; SMAD family member 5; SMAD 5; Smad5; hSmad5

Research area

Signal transduction; Epigenetics and nuclear signaling; Stem cells; Developmental biology

Target protein

SMAD5

Recommended dilution

Application Dilution
FC 1:200 - 1:400
IF 1:200 - 1:1000
IHC 1:200 - 1:1000
WB 1:500 - 1:2000

Images

Western Blot analysis using Smad5 Monoclonal antibody against HeLa (1) SK-N-SH (2) PC-12 (3) Jurkat (4) and K562 (5) cell lysate.
Immunohistochemistry analysis of paraffin-embedded brain tissues (left) and lung cancer tissues (right) with DAB staining using Smad5 Monoclonal antibody.
Immunofluorescence analysis of NTERA-2 cells using Smad5 Monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric analysis of Jurkat cells using Smad5 Monoclonal antibody (green) and negative control (purple).

Protocols

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Product size

Price

  • 50ul

    $180.00

  • 100ul

    $300.00

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Lead time: Within one week

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