Beta-tubulin (HRP Conjugated) Monoclonal Antibody(5G3)

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Home > Product > Antibody > Beta-tubulin (HRP Conjugated) Monoclonal Antibody(5G3)

Beta-tubulin (HRP Conjugated) Monoclonal Antibody(5G3)

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, sheep, Insect, Yeast

Applications: WB, IF, ICC, IHC-p

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Rat,Mouse,Monkey,Dog,Chicken,Hamster,Rabbit,sheep,Insect,Yeast

Applications: WB, IF, ICC, IHC-p

Product overview

Full product name

Tubulin beta chain

Code

BT-MCA1343

Host

Mouse

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Monoclonal

Immunogen

Recombinant Protein of Beta-tubulin (HRP Conjugated)

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13.

Uniprot accession

Human:;Mouse:;Rat:;

Molecular weight

N/A

Gene ID

Human: 203068

Synonyms

Tubulin beta chain; Tubulin beta-5 chain

Research area

Signal transduction; Tags & cell markers

Target protein

TUBB

Recommended dilution

Application	Dilution
IF	1:200
IHC	1:50-1:200
WB	1:1000-1:3000

Images

Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1.Beta-tubulin (HRP Conjugated) Monoclonal antibody(5G3) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1.Beta-tubulin (HRP Conjugated) Monoclonal antibody(5G3) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1.Beta-tubulin (HRP Conjugated) Monoclonal antibody(5G3) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunofluorescence analysis of Human-lung-cancer tissue. 1.Beta-tubulin (HRP Conjugated) Monoclonal antibody(5G3)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of Mouse-testis tissue. 1.Beta-tubulin (HRP Conjugated) Monoclonal antibody(5G3)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of Rat-kidney tissue. 1.Beta-tubulin (HRP Conjugated) Monoclonal antibody(5G3)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Western blot analysis of Hela, with Beta-tubulin-HRP Conjugated diluted at 1:10,000, 20,000, 40,000, 80,000, 160,000, 320,000.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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