Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,AMPKα1/2 Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1,AMPKα1/2 Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-colon tissue. 1,AMPKα1/2 Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°C overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using AMPK alpha Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from 1) K562 , 2) COS7 , 3) KB cells, (Green) primary antibody was diluted at 1:1000, 4°C overnight, secondary antibody was diluted at 1:10000, 37°C 1hour. (Red) GAPDH Monoclonal Antibody(2B8) was diluted at 1:5000 as loading control, 4°C overnight,secondary antibody was diluted at 1:10000, 37°C 1hour.
Western Blot analysis of various cells using AMPKα1/2 Polyclonal Antibody diluted at 1:500
Western Blot analysis of COS7 cells using AMPKα1/2 Polyclonal Antibody diluted at 1:500
Western blot analysis of lysates from COS7 cells, treated with Adriamycin 0.5ng/ml 24h, using AMPK alpha Antibody. The lane on the right is blocked with the synthesized peptide.
