Cortactin(Phospho Tyr466) Polyclonal Antibody

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Home > Product > Antibody > Cortactin(Phospho Tyr466) Polyclonal Antibody

Cortactin(Phospho Tyr466) Polyclonal Antibody

Product Summary

Size: 100ul,50ul,20ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat, Monkey

Applications: WB, IHC-p, IF, ICC, ELISA

Product Summary

Size: 100ul,50ul,20ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat, Monkey

Applications: WB, IHC-p, IF, ICC, ELISA

Product overview

Full product name

Src substrate cortactin

Code

BT-AP02066

Host

Rabbit

Isotype

IgG

Size

100ul,50ul,20ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from mouse Cortactin around the phosphorylation site of Tyr466. AA range:441-490

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for this gene.

Uniprot accession

Human:;Mouse:;

Molecular weight

61636

Gene ID

Human: 13043; Mouse: 13043

Synonyms

Src substrate cortactin; CTTN; EMS1; Src substrate cortactin; Amplaxin; Oncogene EMS1

Research area

Cancer; Signal transduction

Target protein

CTTN

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC-p	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ICC	1: 200 - 1: 1000
ELISA	1: 20000

Images

Immunofluorescence analysis of Hela cell. 1,Cortactin (phospho Tyr466) Polyclonal Antibody(Green) was diluted at 1:200(4°C overnight). 2 DAPI(blue) 10min.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Cortactin (Phospho-Tyr466) Antibody. The picture on the right is blocked with the phospho peptide.

Western Blot analysis of various cells using Phospho-Cortactin (Y466) Polyclonal Antibody diluted at 1:500

Western Blot analysis of 293 cells using Phospho-Cortactin (Y466) Polyclonal Antibody diluted at 1:500

Western blot analysis of lysates from 293 cells, using Cortactin (Phospho-Tyr466) Antibody. The lane on the right is blocked with the phospho peptide.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100ul

$275.00
50ul

$180.00
20ul

$108.00

Add to Cart

Lead time: Within one week

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