Cytokeratin 8(Phospho Ser432) Polyclonal Antibody

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Home > Product > Antibody > Cytokeratin 8(Phospho Ser432) Polyclonal Antibody

Cytokeratin 8(Phospho Ser432) Polyclonal Antibody

Product Summary

Size: 100ul,50ul,20ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, IF, ICC, ELISA

Product Summary

Size: 100ul,50ul,20ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, IF, ICC, ELISA

Product overview

Full product name

Keratin type II cytoskeletal 8

Code

BT-AP05862

Host

Rabbit

Isotype

IgG

Size

100ul,50ul,20ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human Keratin 8 around the phosphorylation site of Ser432. AA range:401-450

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene.

Uniprot accession

Human:;Mouse:;Rat:;

Molecular weight

53573

Gene ID

Human: 3856; Mouse: 16691; Rat: 25626

Synonyms

Keratin type II cytoskeletal 8; KRT8; CYK8; Keratin; type II cytoskeletal 8; Cytokeratin-8; CK-8; Keratin-8; K8; Type-II keratin Kb8

Research area

Signal transduction; Cancer

Target protein

KRT8

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC-p	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ICC	1: 200 - 1: 1000
ELISA	1: 40000

Images

Immunofluorescence analysis of HeLa cells, using Keratin 8 (Phospho-Ser432) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using Keratin 8 (Phospho-Ser432) Antibody. The picture on the right is blocked with the phospho peptide.

Western Blot analysis of 293 cells using Phospho-Cytokeratin 8 (S432) Polyclonal Antibody diluted at 1:1000

Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 30', using Keratin 8 (Phospho-Ser432) Antibody. The lane on the right is blocked with the phospho peptide.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100ul

$275.00
50ul

$180.00
20ul

$108.00

Add to Cart

Lead time: Within one week

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