EGFR(Phospho Tyr1016) Polyclonal Antibody

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Home > Product > Antibody > EGFR(Phospho Tyr1016) Polyclonal Antibody

EGFR(Phospho Tyr1016) Polyclonal Antibody

Product Summary

Size: 100ul,50ul,20ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, IF, ICC, ELISA

Product Summary

Size: 100ul,50ul,20ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, IF, ICC, ELISA

Product overview

Full product name

Epidermal growth factor receptor

Code

BT-AP00569

Host

Rabbit

Isotype

IgG

Size

100ul,50ul,20ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human EGFR around the phosphorylation site of Tyr1016. AA range:986-1035

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.

Uniprot accession

Human:;Mouse:;

Molecular weight

134277

Gene ID

Human: 1956; Mouse: 13649

Synonyms

Epidermal growth factor receptor; EGFR; ERBB; ERBB1; HER1; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

Research area

Cancer; Cell biology; Microbiology; Signal transduction; Epigenetics and nuclear signaling

Target protein

EGFR

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC-p	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ICC	1: 200 - 1: 1000
ELISA	1: 5000

Images

Immunofluorescence analysis of HeLa cells, using EGFR (Phospho-Tyr1016) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°C overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using EGFR (Phospho-Tyr1016) Antibody. The picture on the right is blocked with the phospho peptide.

Western Blot analysis of HELA cells using Phospho-EGFR (Y1016) Polyclonal Antibody diluted at 1:1000

Western blot analysis of lysates from HUVEC cells treated with Serum 20% 30', using EGFR (Phospho-Tyr1016) Antibody. The lane on the right is blocked with the phospho peptide.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100ul

$275.00
50ul

$180.00
20ul

$108.00

Add to Cart

Lead time: Within one week

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