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Home > Product > Antibody > GRF-1(Phospho Tyr1087) Polyclonal Antibody

GRF-1(Phospho Tyr1087) Polyclonal Antibody

GRF-1(Phospho Tyr1087) Polyclonal Antibody

Product Summary

Size: 100ul,50ul,20ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, ELISA

Product Summary

Size: 100ul,50ul,20ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ELISA

Product overview

Full product name

Rho GTPase-activating protein 35

Code

BT-AP06527

Host

Rabbit

Isotype

IgG

Size

100ul,50ul,20ul

Clonality

Polyclonal

Immunogen

Synthesized phospho-peptide around the phosphorylation site of human GRF-1 (phospho Tyr1087)

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

The human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. The amino acid sequence deduced from the cDNA sequences show the presence of three sequence motifs characteristic of a zinc finger and one motif suggestive of a leucine zipper in which 1 cysteine is found instead of all leucines. The GRLF1 enhances the homologous down-regulation of wild-type hGR gene expression. Biochemical analysis suggests that GRLF1 interaction is sequence specific and that transcriptional efficacy of GRLF1 is regulated through its interaction with specific sequence motif. The level of expression is regulated by glucocorticoids.

Uniprot accession

Molecular weight

172229

Gene ID

Human: 2909; Mouse: 232906

Synonyms

Rho GTPase-activating protein 35; ARHGAP35; GRF1; GRLF1; KIAA1722; Rho GTPase-activating protein 35; Glucocorticoid receptor DNA-binding factor 1; Glucocorticoid receptor repression factor 1; GRF-1; Rho GAP p190A; p190-A

Research area

Epigenetics and nuclear signaling

Target protein

ARHGAP35

Recommended dilution

Application Dilution
WB 1: 500 - 1: 2000
ELISA 1: 10000

Images

Western Blot analysis of 293T cells using Phospho-GRF-1 (Y1087) Polyclonal Antibody diluted at 1:1000
Western blot analysis of lysates from K562 cells, using phospho-GRF-1 (Phospho-Tyr1087) antibody.

Protocols

Customer reviews and Q&As

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Product size

Price

  • 100ul

    $275.00

  • 50ul

    $180.00

  • 20ul

    $108.00

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Lead time: Within one week

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