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Home > Product > Antibody > HIRA(Phospho Thr555) Polyclonal Antibody

HIRA(Phospho Thr555) Polyclonal Antibody

HIRA(Phospho Thr555) Polyclonal Antibody

Product Summary

Size: 100ul,50ul,20ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse

Applications: WB, IHC-p, IF, ICC, ELISA

Product Summary

Size: 100ul,50ul,20ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC-p, IF, ICC, ELISA

Product overview

Full product name

Protein HIRA

Code

BT-AP06623

Host

Rabbit

Isotype

IgG

Size

100ul,50ul,20ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human HIRA around the phosphorylation site of Thr555. AA range:521-570

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

This gene encodes a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this gene in yeast, flies, and plants are necessary for the formation of transcriptionally silent heterochomatin. This gene plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate gene in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development.

Uniprot accession

Molecular weight

111835

Gene ID

Human: 7290; Mouse: 15260

Synonyms

Protein HIRA; HIRA; DGCR1; HIR; TUPLE1; Protein HIRA; TUP1-like enhancer of split protein 1

Research area

Stem cells; Cancer; Cardiovascular; Metabolism; Neuroscience; Signal transduction; Developmental biology

Target protein

HIRA

Recommended dilution

Application Dilution
IHC-p 1: 100 - 1: 300
IF 1: 200 - 1: 1000
ICC 1: 200 - 1: 1000
ELISA 1: 10000

Images

Immunofluorescence analysis of HeLa cells, using HIRA (Phospho-Thr555) Antibody. The picture on the right is blocked with the phospho peptide.
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4°C overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Protocols

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Product size

Price

  • 100ul

    $275.00

  • 50ul

    $180.00

  • 20ul

    $108.00

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Lead time: Within one week

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