His-tag Polyclonal Antibody

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Blood coagulation
Metabolism
Cancer
Microbiology
Cardiovascular
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Cell biology
Signal transduction
Developmental biology
Stem cells
Epigenetics and nuclear signaling
Tags & cell markers
Immunology

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Home > Product > Antibody > His-tag Polyclonal Antibody

His-tag Polyclonal Antibody

Product Summary

Size: 100ul,50uL,20ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Species independent

Applications: WB, ELISA

Product Summary

Size: 100ul,50uL,20ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Species independent

Applications: WB, ELISA

Product overview

Full product name

His Tag

Code

BT-AP00862

Host

Rabbit

Isotype

IgG

Size

100ul,50uL,20ul

Clonality

Polyclonal

Immunogen

6X His synthetic peptide conjugated to KLH.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.05% sodium azide.

Storage

-20°C for 1 year

Background

The his-tag is a series of six to nine histidine residues generally fused to either the carboxy or amino terminus of a recombinant protein. The small size of the his-tag, compared with other common epitope tags, makes it less likely to obstruct the target protein’s structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions, allows for the simple purification and detection of his-tagged proteins.

Synonyms

His Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag

Research area

Tags & cell markers ; Signal transduction; Epigenetics and nuclear signaling

Target protein

His-Tag

Recommended dilution

Application	Dilution
WB	1: 1000 - 1: 3000
ELISA	1: 20000

Images

Western Blot analysis using His-tag Polyclonal Antibody against HEK293 cells transfected with vector overexpressing His tag (1) and untransfected (2). Antibody was diluted at 1:2000. Secondary antibody was diluted at 1:20000

Western Blot analysis of HIS-protein cells using His-tag Polyclonal Antibody diluted at 1:2000. Secondary antibody was diluted at 1:20000

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100ul

$275.00
50uL

$180.00
20ul

$108.00

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Lead time: Within one week

Custom quote | Bulk request

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