Histone H3(Phospho Ser10) Polyclonal Antibody

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Blood coagulation
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Epigenetics and nuclear signaling
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Home > Product > Antibody > Histone H3(Phospho Ser10) Polyclonal Antibody

Histone H3(Phospho Ser10) Polyclonal Antibody

Product Summary

Size: 100ul,50ul,20ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, IHC-f, Flow Cyt, ICC, IF, ICC, ELISA

Product Summary

Size: 100ul,50ul,20ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, IHC-f, Flow Cyt, ICC, IF, ICC, ELISA

Product overview

Full product name

Histone H3.1

Code

BT-AP06657

Host

Rabbit

Isotype

IgG

Size

100ul,50ul,20ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human Histone H3.1 around the phosphorylation site of Ser10. AA range:1-50

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

Uniprot accession

Human:;Rat:;

Molecular weight

15273

Gene ID

Human: 8350/8351/8352/8353/8354/8355/8356/8357/8358/8968; Mouse: 319152/15077/15078; Rat: 291159/100361558

Synonyms

Histone H3.1; HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3

Research area

Epigenetics and nuclear signaling; Signal transduction; Tags & cell markers

Target protein

HIST1H3A

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC-f	1: 500
IHC	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ICC	1: 200 - 1: 1000
Flow Cyt	1: 500
ELISA	1: 10000

Images

Immunofluorescence analysis of Hela cell. Histone H3 (phospho Ser10) Polyclonal Antibody(Red) was diluted at 1:200(4°C overnight). β-Tubulin Monoclonal Antibody(5G3)(Green) was diluted at 1:200(4°C overnight).

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Histone H3.1 (Phospho-Ser10) Antibody. The picture on the right is blocked with the phospho peptide.

Western Blot analysis of various cells using Phospho-Histone H3 (S10) Polyclonal Antibody

Western Blot analysis of KB cells using Phospho-Histone H3 (S10) Polyclonal Antibody

Western blot analysis of lysates from NIH/3T3 cells and HepG2 cells, using Histone H3.1 (Phospho-Ser10) Antibody. The lane on the right is blocked with the phospho peptide.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100ul

$275.00
50ul

$180.00
20ul

$108.00

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Lead time: Within one week

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