MDM2(Phospho Ser166) Polyclonal Antibody

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Home > Product > Antibody > MDM2(Phospho Ser166) Polyclonal Antibody

MDM2(Phospho Ser166) Polyclonal Antibody

Product Summary

Size: 100ul,50ul,20ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Monkey

Applications: WB, IHC-p, IF, ICC, ELISA

Product Summary

Size: 100ul,50ul,20ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Monkey

Applications: WB, IHC-p, IF, ICC, ELISA

Product overview

Full product name

E3 ubiquitin-protein ligase Mdm2

Code

BT-AP06974

Host

Rabbit

Isotype

IgG

Size

100ul,50ul,20ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human MDM2 around the phosphorylation site of Ser166. AA range:132-181

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells.

Uniprot accession

Human:;Mouse:;

Molecular weight

55233

Gene ID

Human: 4193; Mouse: 17246

Synonyms

E3 ubiquitin-protein ligase Mdm2; MDM2; E3 ubiquitin-protein ligase Mdm2; Double minute 2 protein; Hdm2; Oncoprotein Mdm2; p53-binding protein Mdm2

Research area

Cancer; Cell biology; Epigenetics and nuclear signaling

Target protein

MDM2

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC-p	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ICC	1: 200 - 1: 1000
ELISA	1: 5000

Images

Immunofluorescence analysis of Hela cell. MDM2 (phospho Ser166) Polyclonal Antibody(Red) was diluted at 1:200(4°C overnight). HAO1 Monoclonal Antibody(Mix)(Green) was diluted at 1:200(4°C overnight).

Immunofluorescence analysis of A549 cells, using MDM2 (Phospho-Ser166) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MDM2 (Phospho-Ser166) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from COS7 cells, using MDM2 (Phospho-Ser166) Antibody. The lane on the right is blocked with the phospho peptide.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100ul

$275.00
50ul

$180.00
20ul

$108.00

Add to Cart

Lead time: Within one week

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