Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1,NFκB-p65 (phospho Ser536) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1,NFκB-p65 (phospho Ser536) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-Appendix tissue. 1,NFκB-p65 (phospho Ser536) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1,NFκB-p65 (phospho Ser536) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,NFκB-p65 (phospho Ser536) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NF-kappaB p65 (Phospho-Ser536) Antibody. The picture on the right is blocked with the phospho peptide.
Western Blot analysis of various cells using Phospho-NFκB-p65 (S536) Polyclonal Antibody diluted at 1:2000
Western Blot analysis of COLO205 cells using Phospho-NFκB-p65 (S536) Polyclonal Antibody diluted at 1:2000
Western Blot analysis of A549 3T3 293T K562 cells using Antibody diluted at 2000. Secondary antibody was diluted at 1:20000
Western blot analysis of lysates from K562 cells and COLO cells, using NF-kappaB p65 (Phospho-Ser536) Antibody. The lane on the right is blocked with the phospho peptide.
Western blot analysis of lysates from 1) A549, 2) 3T3, 3) 293T , 4)K562 cells, (Green) primary antibody was diluted at 1:1000, 4°C overnight, secondary antibody was diluted at 1:10000, 37°C 1hour. (Red) Actin β Monoclonal Antibody(5B7) was diluted at 1:5000 as loading control, 4°C overnight,secondary antibody was diluted at 1:10000, 37°C 1hour.
