PKC Zeta (Phospho Thr560) Polyclonal Antibody

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Home > Product > Antibody > PKC Zeta (Phospho Thr560) Polyclonal Antibody

PKC Zeta (Phospho Thr560) Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat, Monkey

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat, Monkey

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

Protein kinase C zeta type

Code

BT-AP07586

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human PKC zeta around the phosphorylation site of Thr560. AA range:526-575

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms.

Uniprot accession

Human:;Mouse:;Rat:;

Molecular weight

67660

Gene ID

Human: 5590; Mouse: 18762; Rat: 25522

Synonyms

Protein kinase C zeta type; PRKCZ; PKC2; Protein kinase C zeta type; nPKC-zeta

Research area

Signal transduction

Target protein

PRKCZ

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC-p	1: 100 - 1: 300
ELISA	1: 20000

Images

Immunohistochemistry analysis of paraffin-embedded human brain, using PKC zeta (Phospho-Thr560) Antibody. The picture on the right is blocked with the phospho peptide.

Western Blot analysis of HELA 293T cells using Phospho-PKC ζ (T560) Polyclonal Antibody diluted at 1:2000

Western blot analysis of lysates from COS7 cells treated with PMA 125ng/ml 30', using PKC zeta (Phospho-Thr560) Antibody. The lane on the right is blocked with the phospho peptide.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

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Lead time: Within one week

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