Separase(Phospho Ser801) Polyclonal Antibody

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Home > Product > Antibody > Separase(Phospho Ser801) Polyclonal Antibody

Separase(Phospho Ser801) Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse

Applications: WB, IHC-p, IF, ICC, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC-p, IF, ICC, ELISA

Product overview

Full product name

Separin

Code

BT-AP07944

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human SEPARASE around the phosphorylation site of Ser801. AA range:767-816

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates| sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1| or separase| which initiates the final separation of sister chromatids (Sun et al.| 2009

Uniprot accession

Human:;Mouse:;

Molecular weight

233113

Gene ID

Human: 9700; Mouse: 105988

Synonyms

Separin; ESPL1; ESP1; KIAA0165; Separin; Caspase-like protein ESPL1; Extra spindle poles-like 1 protein; Separase

Research area

Cell biology; Epigenetics and nuclear signaling

Target protein

ESPL1

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC-p	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ICC	1: 200 - 1: 1000
ELISA	1: 40000

Images

Immunofluorescence analysis of HUVEC cells, using SEPARASE (Phospho-Ser801) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using SEPARASE (Phospho-Ser801) Antibody. The picture on the right is blocked with the phospho peptide.

Western Blot analysis of various cells using Phospho-Separase (S801) Polyclonal Antibody diluted at 1:1000

Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 30', using SEPARASE (Phospho-Ser801) Antibody. The lane on the right is blocked with the phospho peptide.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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