SGK1(Phospho Ser422) Polyclonal Antibody

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Blood coagulation
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Home > Product > Antibody > SGK1(Phospho Ser422) Polyclonal Antibody

SGK1(Phospho Ser422) Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

Serine/threonine-protein kinase Sgk1

Code

BT-AP07948

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human SGK around the phosphorylation site of Ser422. AA range:381-430

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

This gene encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium| sodium| and chloride channels| suggesting an involvement in the regulation of processes such as cell survival| neuronal excitability| and renal sodium excretion. High levels of expression of this gene may contribute to conditions such as hypertension and diabetic nephropathy. Several alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

Uniprot accession

Human:;Mouse:;Rat:;

Molecular weight

48942

Gene ID

Human: 6446; Mouse: 20393

Synonyms

Serine/threonine-protein kinase Sgk1; SGK1; SGK; Serine/threonine-protein kinase Sgk1; Serum/glucocorticoid-regulated kinase 1

Research area

Developmental biology; Signal transduction; Stem cells

Target protein

SGK1

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC-p	1: 100 - 1: 300
ELISA	1: 40000

Images

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°C overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using SGK (Phospho-Ser422) Antibody. The picture on the right is blocked with the phospho peptide.

Western Blot analysis of VEC HY926 cells using Phospho-SGK1 (S422) Polyclonal Antibody diluted at 1:500

Western blot analysis of lysates from HeLa cells treated with Insulin 0.01U/ml 15', using SGK (Phospho-Ser422) Antibody. The lane on the right is blocked with the phospho peptide.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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