Splicing factor 1(Phospho Ser82) Polyclonal Antibody

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Home > Product > Antibody > Splicing factor 1(Phospho Ser82) Polyclonal Antibody

Splicing factor 1(Phospho Ser82) Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Monkey

Applications: WB, IHC-p, IF, ICC, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Monkey

Applications: WB, IHC-p, IF, ICC, ELISA

Product overview

Full product name

Splicing factor 1

Code

BT-AP01624

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human SF1 around the phosphorylation site of Ser82. AA range:48-97

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site| together with the large subunit of U2 auxiliary factor (U2AF)| and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif| a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence| a zinc knuckles domain| and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants.

Uniprot accession

Human:;Mouse:;

Molecular weight

68330

Gene ID

Human: 7536

Synonyms

Splicing factor 1; SF1; ZFM1; ZNF162; Splicing factor 1; Mammalian branch point-binding protein; BBP; mBBP; Transcription factor ZFM1; Zinc finger gene in MEN1 locus; Zinc finger protein 162

Research area

Developmental biology; Epigenetics and nuclear signaling

Target protein

SF1

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC-p	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ICC	1: 200 - 1: 1000
ELISA	1: 5000

Images

Immunofluorescence analysis of A549 cells, using SF1 (Phospho-Ser82) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human brain, using SF1 (Phospho-Ser82) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of SH-SY5Y 293T using p-Splicing factor 1 (S82) antibody. Antibody was diluted at 1:500

Western blot analysis of lysates from HUVEC cells treated with anisomycin 25ug/ml 30', using SF1 (Phospho-Ser82) Antibody. The lane on the right is blocked with the phospho peptide.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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