Immunofluorescence analysis of Hela cell. Stat1 (phospho Tyr701) Polyclonal Antibody(Red) was diluted at 1:200(4°C overnight). HER2 Monoclonal Antibody(11H9)(Green) was diluted at 1:200(4°C overnight).
Immunofluorescence analysis of rat-spleen tissue. 1,Stat1 (phospho Tyr701) Polyclonal Antibody(Red) was diluted at 1:200(4°C overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of rat-spleen tissue. 1,Stat1 (phospho Tyr701) Polyclonal Antibody(Red) was diluted at 1:200(4°C overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of mouse-spleen tissue. 1,Stat1 (phospho Tyr701) Polyclonal Antibody(Red) was diluted at 1:200(4°C overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of mouse-spleen tissue. 1,Stat1 (phospho Tyr701) Polyclonal Antibody(Red) was diluted at 1:200(4°C overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,Stat1 (phospho Tyr701) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1,Caspase-1 Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-spleen tissue. 1,Stat1 (phospho Tyr701) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using STAT1 (Phospho-Tyr701) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from 1) MOUSE-BRAIN, 2) SY-SY5X ,3) COS7 cells, (Green) primary antibody was diluted at 1:1000, 4°C overnight, secondary antibody was diluted at 1:10000, 37°C 1hour. (Red) Actin β Monoclonal Antibody(5B7) was diluted at 1:5000 as loading control, 4°C overnight,secondary antibody was diluted at 1:10000, 37°C 1hour.
Western Blot analysis of various cells using Phospho-Stat1 (Y701) Polyclonal Antibody diluted at 1:1000
Western Blot analysis of COS7 cells using Phospho-Stat1 (Y701) Polyclonal Antibody diluted at 1:1000
Western blot analysis of lysates from COS7 cells, using STAT1 (Phospho-Tyr701) Antibody. The lane on the right is blocked with the phospho peptide.
