Immunofluorescence analysis of Hela cell. Stat3 (phospho Ser727) Polyclonal Antibody(Red) was diluted at 1:200(4°C overnight). Caspase 9 Monoclonal Antibody(3-20)(Green) was diluted at 1:200(4°C overnight).
Immunofluorescence analysis of rat-heart tissue. 1,Stat3 (phospho Ser727) Polyclonal Antibody(Red) was diluted at 1:200(4°C overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of rat-heart tissue. 1,Stat3 (phospho Ser727) Polyclonal Antibody(Red) was diluted at 1:200(4°C overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,PDGF-B Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1,Stat3 (phospho Ser727) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,Stat3 (phospho Ser727) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,Stat3 (phospho Ser727) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1,Stat3 (phospho Ser727) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1,Stat3 (phospho Ser727) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,Stat3 (phospho Ser727) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,Stat3 (phospho Ser727) Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using STAT3 (Phospho-Ser727) Antibody. The picture on the right is blocked with the phospho peptide.
Western Blot analysis of various cells using Phospho-Stat3 (S727) Polyclonal Antibody diluted at 1:2000
Western Blot analysis of COS7 cells using Phospho-Stat3 (S727) Polyclonal Antibody diluted at 1:2000
Western blot analysis of lysates from COS7 cells, using STAT3 (Phospho-Ser727) Antibody. The lane on the right is blocked with the phospho peptide.
Western blot analysis of lysates from COS7,Jurkat cells, treated or untreated with TNFa, (Green)primary antibody was diluted at 1:1000, 4°C overnight, secondary antibody was diluted at 1:10000, 37°C 1hour. (Red) loading contrl antibody was diluted at 1:5000 as loading control, 4°C overnight,secondary antibody was diluted at 1:10000, 37°C 1hour.
