Bax Polyclonal Antibody

Product

OneStep ELISA kit
Primacu ELISA kit
Antibodies
Biochemicals
Recombinant proteins

Service

Custom ELISA assay services
Bulk quote

Research area

Blood coagulation
Metabolism
Cancer
Microbiology
Cardiovascular
Neuroscience
Cell biology
Signal transduction
Developmental biology
Stem cells
Epigenetics and nuclear signaling
Tags & cell markers
Immunology

Resources

Protocols
FAQs
Product brochure
Promotion
Troubleshooting guides
About ELISAs

About us

Company profile
Worldwide distributors
Manufacturing service

Home > Product > Antibody > Bax Polyclonal Antibody

Bax Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

Apoptosis regulator BAX

Code

BT-AP06693

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human Bax. AA range:1-50

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

The protein encoded by BAX (BCL2 associated X, apoptosis regulator) belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for BAX.

Uniprot accession

Human:;Mouse:;Rat:;

Molecular weight

21184

Gene ID

Human: 581; Mouse: 12028

Synonyms

Apoptosis regulator BAX; BAX; BCL2L4; Apoptosis regulator BAX; Bcl-2-like protein 4; Bcl2-L-4

Research area

Cancer; Cell biology; Metabolism

Target protein

BAX

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC-p	1: 100 - 1: 300
ELISA	1: 10000

Images

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Bax Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from 1) Hela , 2) MCF7 , 3) k562 , 4) A549 cells, (Green) primary antibody was diluted at 1:1000, 4°C overnight, secondary antibody was diluted at 1:10000, 37°C 1hour. (Red) Tubulin β Monoclonal Antibody(5G3) was diluted at 1:5000 as loading control, 4°C overnight,secondary antibody was diluted at 1:10000, 37°C 1hour.

Western blot analysis of Bax Polyclonal Antibody, using Hela, MCF7,k562,A549 cell, 4°C overnight, secondary antibody was diluted at 1:10000, 37°C 1hour.

Western Blot analysis of various cells using Bax Polyclonal Antibody diluted at 1:2000

Western Blot analysis of HeLa cells using Bax Polyclonal Antibody diluted at 1:2000

Western Blot analysis of 293T using Bax Polyclonal Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000

Western blot analysis of various cell Lysate, antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Western blot analysis of lysates from HeLa cells, using Bax Antibody. The lane on the right is blocked with the synthesized peptide.

Protocols

> Protocol Booklet

Customer reviews and Q&As

No reviews

Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

Custom quote | Bulk request

We are continuously updating our Antibody catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.