Immunofluorescence analysis of HeLa cells, using PECAM-1 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded Human lung. 1, Antibody was diluted at 1:200(4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human lung. 1, Antibody was diluted at 1:400(4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human lung. 1, Antibody was diluted at 1:400(4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:400(4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:400(4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:400(4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using PECAM-1 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from 1) HepG2, 2) K562,3) L929,4) MOUSE-BRAIN cells, (Green) primary antibody was diluted at 1:1000, 4°C overnight, secondary antibody was diluted at 1:10000, 37°C 1hour. (Red) Actin β Monoclonal Antibody(5B7) antibody was diluted at 1:5000 as loading control, 4°C overnight,secondary antibody was diluted at 1:10000, 37°C 1hour.
Western Blot analysis of various cells using CD31 Polyclonal Antibody diluted at 1:1000
Western Blot analysis of rat-musle cells using CD31 Polyclonal Antibody diluted at 1:1000
Western blot analysis of KB lysis using CD31 antibody. Antibody was diluted at 1:1000
Western blot analysis of 293T lysis using CD31 antibody. Antibody was diluted at 1:1000
Western blot analysis of lysates from Jurkat cells, using PECAM-1 Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of the lysates from HT-29 cells using PECAM-1 antibody.
