Cdc25B(Phospho Ser353) Polyclonal Antibody

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Blood coagulation
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Epigenetics and nuclear signaling
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Home > Product > Antibody > Cdc25B(Phospho Ser353) Polyclonal Antibody

Cdc25B(Phospho Ser353) Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Monkey

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Monkey

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

M-phase inducer phosphatase 2

Code

BT-AP07495

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human CDC25B around the phosphorylation site of Ser353. AA range:319-368

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist.

Uniprot accession

Human:;Mouse:;

Molecular weight

64987

Gene ID

Human: 994

Synonyms

M-phase inducer phosphatase 2; CDC25B; CDC25HU2; M-phase inducer phosphatase 2; Dual specificity phosphatase Cdc25B

Research area

Cancer; Cell biology; Epigenetics and nuclear signaling

Target protein

CDC25B

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC-p	1: 100 - 1: 300
ELISA	1: 40000

Images

Immunohistochemistry analysis of paraffin-embedded human brain, using CDC25B (Phospho-Ser353) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from COS7 cells treated with etoposide 25uM 24h, using CDC25B (Phospho-Ser353) Antibody. The lane on the right is blocked with the phospho peptide.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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