Crk II(Phospho Tyr221) Polyclonal Antibody

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Home > Product > Antibody > Crk II(Phospho Tyr221) Polyclonal Antibody

Crk II(Phospho Tyr221) Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat, Monkey

Applications: WB, IF, ICC, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat, Monkey

Applications: WB, IF, ICC, ELISA

Product overview

Full product name

Adapter molecule crk

Code

BT-AP08108

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human CrkII around the phosphorylation site of Tyr221. AA range:187-236

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described.

Uniprot accession

Human:;Mouse:;Rat:;

Molecular weight

33872

Gene ID

Human: 1398; Mouse: 12928; Rat: 54245

Synonyms

Adapter molecule crk; CRK; Adapter molecule crk; Proto-oncogene c-Crk; p38

Research area

Cancer; Signal transduction

Target protein

CRK

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IF	1: 200 - 1: 1000
ICC	1: 200 - 1: 1000
ELISA	1: 40000

Images

Immunofluorescence analysis of HUVEC cells, using CrkII (Phospho-Tyr221) Antibody. The picture on the right is blocked with the phospho peptide.

Western Blot analysis of various cells using Phospho-Crk II (Y221) Polyclonal Antibody

Western blot analysis of lysates from COS7 cells, using CrkII (Phospho-Tyr221) Antibody. The lane on the right is blocked with the phospho peptide.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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