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Home > Product > Antibody > Cyclin E1(Phospho Thr395) Polyclonal Antibody

Cyclin E1(Phospho Thr395) Polyclonal Antibody

Cyclin E1(Phospho Thr395) Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Rat, Mouse

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Rat, Mouse

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

G1/S-specific cyclin-E1

Code

BT-AP15144

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human Cyclin E1 around the phosphorylation site of Thr395. AA range:361-410

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in

Uniprot accession

Molecular weight

47077

Gene ID

Human: 898

Synonyms

G1/S-specific cyclin-E1; CCNE1; CCNE; G1/S-specific cyclin-E1

Research area

Cancer; Cell biology; Epigenetics and nuclear signaling

Target protein

CCNE1

Recommended dilution

Application Dilution
WB 1: 500 - 1: 2000
IHC-p 1: 100 - 1: 300
ELISA 1: 10000

Images

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using Cyclin E1 (Phospho-Thr395) Antibody. The picture on the right is blocked with the phospho peptide.
Western Blot analysis of various cells using Phospho-Cyclin E1 (T395) Polyclonal Antibody diluted at 1:2000
Western Blot analysis of HeLa cells using Phospho-Cyclin E1 (T395) Polyclonal Antibody diluted at 1:2000
Western blot analysis of lysates from HeLa cells treated with Paclitaxel 1uM 60', using Cyclin E1 (Phospho-Thr395) Antibody. The lane on the right is blocked with the phospho peptide.

Protocols

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Product size

Price

  • 20ul

    $108.00

  • 50ul

    $180.00

  • 100ul

    $275.00

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Lead time: Within one week

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