eIF4E(Phospho Ser209) Polyclonal Antibody

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Home > Product > Antibody > eIF4E(Phospho Ser209) Polyclonal Antibody

eIF4E(Phospho Ser209) Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

Eukaryotic translation initiation factor 4E

Code

BT-AP08788

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human eIF4E around the phosphorylation site of Ser209. AA range:168-217

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants.

Uniprot accession

Human:;Mouse:;Rat:;

Molecular weight

25097

Gene ID

Human: 1977; Mouse: 13684; Rat: 117045

Synonyms

Eukaryotic translation initiation factor 4E; EIF4E; EIF4EL1; EIF4F; Eukaryotic translation initiation factor 4E; eIF-4E; eIF4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein

Research area

Epigenetics and nuclear signaling

Target protein

EIF4E

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC-p	1: 100 - 1: 300
ELISA	1: 20000

Images

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using eIF4E (Phospho-Ser209) Antibody. The picture on the right is blocked with the phospho peptide.

Western Blot analysis of various cells using Phospho-eIF4E (S209) Polyclonal Antibody

Western blot analysis of lysates from 293 cells treated with Anisomycin 25ug/ml 30', using eIF4E (Phospho-Ser209) Antibody. The lane on the right is blocked with the phospho peptide.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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