Endoglin Polyclonal Antibody

Product overview

Full product name

Endoglin

Code

BT-AP15400

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

Synthetic peptide from human protein at AA range: 370-430

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

This gene encodes a homodimeric transmembrane protein which is a major glycoprotein of the vascular endothelium. This protein is a component of the transforming growth factor beta receptor complex and it binds to the beta1 and beta3 peptides with high affinity. Mutations in this gene cause hereditary hemorrhagic telangiectasia, also known as Osler-Rendu-Weber syndrome 1, an autosomal dominant multisystemic vascular dysplasia. This gene may also be involved in preeclampsia and several types of cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Uniprot accession

Human:;Mouse:;

Gene ID

Human: 2022; Mouse: 13805

Synonyms

Endoglin ;CD antigen CD105; Endoglin; CD antigen CD105

Research area

Immunology; Stem cells; Cancer; Cardiovascular

Target protein

ENG

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC-p	1: 500 - 1: 200
ELISA	1: 20000
ELISA	1: 10000 - 1: 20000

Images

Immunohistochemical analysis of paraffin-embedded human-tonsil, antibody was diluted at 1:200

Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200

Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:200(4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200(4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Western blot analysis of lysates from 1) 3T3 , 2) K562 , 3) Hela cells, (Green) primary antibody was diluted at 1:1000, 4°C overnight, secondary antibody was diluted at 1:10000, 37°C 1hour. (Red) GAPDH Monoclonal Antibody(2B8) was diluted at 1:5000 as loading control, 4°C overnight,secondary antibody was diluted at 1:10000, 37°C 1hour.

Western blot analysis of 3T3 KB K562 Hela 293T lysate, antibody was diluted at 500. Secondary antibody was diluted at 1:20000

Protocols

> Protocol Booklet

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