We use cookies to give you the best experience. By using bt-laboratory.com, you
consent to our use of cookies. If you do not wish to receive our cookies, adjust your browser settings. Read
our Cookies
Policy to learn more.
Reject All
Accept All Cookies
We use cookies to give you the best experience. By using bt-laboratory.com,
you consent to our use of cookies. If you do not wish to receive our cookies, adjust your browser settings.
Read our Cookies
Policy to learn more.
Home >
Product > Antibody > EYFP-Tag Polyclonal Antibody
EYFP-Tag Polyclonal Antibody
EYFP-Tag Polyclonal Antibody
Product Summary
Size:
20ul,50uL,100ul
Clonality: Polyclonal
Host: Mouse
Reactivity:Species independent
Applications: WB
Product Summary
Size:
20ul,50uL,100ul
Clonality: Polyclonal
Host: Mouse
Reactivity: Species independent
Applications: WB
Product overview
Code
BT-AP09022
Host
Mouse
Isotype
IgG
Size
20ul,50uL,100ul
Clonality
Polyclonal
Immunogen
Recombinant Protein of EYFP-Tag
Purification
The antibody was affinity-purified from mouse antiserum by affinity-chromatography using specific immunogen.
Formulation
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Storage
-20°C for 1 year
Background
Enhanced Yellow florescent protein.Yellow Fluorescent Protein (YFP) is a genetic mutant of green fluorescent protein, derived from Aequorea victoria. Its excitation peak is 514nm and its emission peak is 527nm. Like green fluorescent protein (GFP), it is a useful tool in cell and molecular biology, usually explored using fluorescence microscopy. Three improved versions of YFP are Citrine, Venus, and Ypet. They have reduced chloride sensitivity, faster maturation, and increased brightness (product of the extinction coefficient and quantum yield). Typically, yellow FPs serve as the acceptor for genetically-encoded FRET sensors of which the most likely donor FP is mCFP (monomeric cyan FP). The red-shift relative to GFP is caused by a Pi-Pi stacking interaction as a result of the T203Y mutation, which essentially increases the polarizability of the local chromophore environment as well as providing additional electron density into the chromophore.
Research area
Tags & cell markers
Recommended dilution
Application
Dilution
WB
1: 1000 - 1: 3000
Images
Western blot analysis of EYFP Recombinant protein, diluted at 1:2000;1:5000. Secondary antibody was diluted at 1:20000
We are continuously updating our Antibody catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.