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Home > Product > Antibody > EYFP-Tag Polyclonal Antibody

EYFP-Tag Polyclonal Antibody

EYFP-Tag Polyclonal Antibody

Product Summary

Size: 20ul,50uL,100ul

Clonality: Polyclonal

Host: Mouse

Reactivity:Species independent

Applications: WB

Product Summary

Size: 20ul,50uL,100ul

Clonality: Polyclonal

Host: Mouse

Reactivity: Species independent

Applications: WB

Product overview

Code

BT-AP09022

Host

Mouse

Isotype

IgG

Size

20ul,50uL,100ul

Clonality

Polyclonal

Immunogen

Recombinant Protein of EYFP-Tag

Purification

The antibody was affinity-purified from mouse antiserum by affinity-chromatography using specific immunogen.

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Storage

-20°C for 1 year

Background

Enhanced Yellow florescent protein.Yellow Fluorescent Protein (YFP) is a genetic mutant of green fluorescent protein, derived from Aequorea victoria. Its excitation peak is 514nm and its emission peak is 527nm. Like green fluorescent protein (GFP), it is a useful tool in cell and molecular biology, usually explored using fluorescence microscopy. Three improved versions of YFP are Citrine, Venus, and Ypet. They have reduced chloride sensitivity, faster maturation, and increased brightness (product of the extinction coefficient and quantum yield). Typically, yellow FPs serve as the acceptor for genetically-encoded FRET sensors of which the most likely donor FP is mCFP (monomeric cyan FP). The red-shift relative to GFP is caused by a Pi-Pi stacking interaction as a result of the T203Y mutation, which essentially increases the polarizability of the local chromophore environment as well as providing additional electron density into the chromophore.

Research area

Tags & cell markers

Recommended dilution

Application Dilution
WB 1: 1000 - 1: 3000

Images

Western blot analysis of EYFP Recombinant protein, diluted at 1:2000;1:5000. Secondary antibody was diluted at 1:20000

Protocols

Customer reviews and Q&As

No reviews
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Product size

Price

  • 20ul

    $108.00

  • 50uL

    $180.00

  • 100ul

    $275.00

Add to Cart

Lead time: Within one week

We are continuously updating our Antibody catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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