Full product name
Flag-Tag DDDDK TAG, DDDDK-TAG
Code
BT-AP15612
Host
Rabbit
Isotype
IgG
Size
20ul,50uL,100ul
Clonality
Polyclonal
Immunogen
DDDDK synthetic peptide conjugated to KLH.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.05% sodium azide.
Storage
-20°C for 1 year
Background
The DYKDDDDK (FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A FLAG-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.
Synonyms
Flag-Tag DDDDK TAG, DDDDK-TAG; DDDDK epitope tag; DDDDK epitope tag; DYKDDDDK epitope tag
Research area
Tags & cell markers
Target protein
Flag
