Histone H1(Phospho Thr17) Polyclonal Antibody

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Home > Product > Antibody > Histone H1(Phospho Thr17) Polyclonal Antibody

Histone H1(Phospho Thr17) Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Monkey

Applications: WB, IHC-p, IF, ICC, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Monkey

Applications: WB, IHC-p, IF, ICC, ELISA

Product overview

Full product name

Histone H1.5

Code

BT-AP09916

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human Histone H1 around the phosphorylation site of Thr17. AA range:1-50

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.

Uniprot accession

Human:;

Molecular weight

22580

Gene ID

Human: 3009/3007/3008; Mouse: 56702/14957/50709

Synonyms

Histone H1.5; HIST1H1B; H1F5; Histone H1.5; Histone H1a; Histone H1b; Histone H1s-3; HIST1H1D; H1F3; Histone H1.3; Histone H1c; Histone H1s-2; HIST1H1E; H1F4; Histone H1.4; Histone H1b; Histone H1s-4

Research area

Epigenetics and nuclear signaling

Target protein

HIST1H1B

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC-p	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ICC	1: 200 - 1: 1000
ELISA	1: 10000

Images

Immunofluorescence analysis of Hela cell. Histone H1 (phospho Thr17) Polyclonal Antibody(Red) was diluted at 1:200(4°C overnight). NSE Monoclonal Antibody(13E2)(Green) was diluted at 1:200(4°C overnight).

Immunofluorescence analysis of HUVEC cells treated with serum 20% 30', using Histone H1 (Phospho-Thr17) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using Histone H1 (Phospho-Thr17) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of SKOV3 using p-Histone H1 (T17) antibody. Antibody was diluted at 1:1000

Western blot analysis of lysates from Jurkat cells treated with UV 15', using Histone H1 (Phospho-Thr17) Antibody. The lane on the right is blocked with the phospho peptide.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

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Lead time: Within one week

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