Histone H2A.X Polyclonal Antibody

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Home > Product > Antibody > Histone H2A.X Polyclonal Antibody

Histone H2A.X Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Rat, Mouse

Applications: WB, IHC-p, IF, ICC, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Rat, Mouse

Applications: WB, IHC-p, IF, ICC, ELISA

Product overview

Full product name

Histone H2A.x

Code

BT-AP09933

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human Histone H2AX . AA range:94-143

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.

Uniprot accession

Human:;Mouse:;

Molecular weight

15145

Gene ID

Human: 3014

Synonyms

Histone H2A.x; H2AFX; H2AX; Histone H2A.x; H2a/x

Research area

Epigenetics and nuclear signaling

Target protein

H2AFX

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC-p	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ICC	1: 200 - 1: 1000
ELISA	1: 20000

Images

Immunofluorescence analysis of Hela cell. Histone H2A.X Polyclonal Antibody(Red) was diluted at 1:200(4°C overnight). HAO1 Monoclonal Antibody(Mix)(Green) was diluted at 1:200(4°C overnight).

Immunofluorescence analysis of COS7 cells, using Histone H2AX? Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°C overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Western Blot analysis of various cells using Histone H2A.X Polyclonal Antibody diluted at 1:1000

Western blot analysis of lysates from HT-29 cells, using Histone H2AX? Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of the lysates from K562 cells using Histone H2AX? antibody.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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